Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) BioAssay(TM) ELISA Kit (Rat)

Intended Use:, Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) BioAssayT ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of UCHL1 in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 96% homology of the sequence among different species, the kit is expected to crossreact with human and mouse samples. Detection Range: 0.78-50ng/ml, Sensitivity: 0.28ng/ml, Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit Components: *028750A: Microtiter Strips, 1x96 wells, *028750B: Standard, 2x1 vial, 028750C: Standard Diluent, 1x20ml, *028750D: Detection Reagent A (green), 1x120ul, *028750E: Detection Reagent B (red), 1x120ul, 028750F: Assay Diluent A, 1x12ml, 028750G: Assay Diluent B, 1x12ml, 028750H: TMB Substrate, 1x9ml, 028750K: Stop Solution, 1x6ml, 028750L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (028750K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *028750A, *028750B, *028750D and *028750E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.