Tumor Necrosis Factor alpha (TNFa) BioAssay(TM) ELISA Kit (Human)

Tumor Necrosis Factor alpha (TNFa) BioAssay(TM) ELISA Kit (Human)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
028669.96 96 tests - -

3 - 19 business days*

789.00€
 
Tumor Necrosis Factor alpha (TNFa) BioAssayT ELISA Kit (Human) is a sandwich enzyme immunoassay... more
Product information "Tumor Necrosis Factor alpha (TNFa) BioAssay(TM) ELISA Kit (Human)"
Tumor Necrosis Factor alpha (TNFa) BioAssayT ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in human serum, plasma, tissue homogenates, cell lysates, cell cilture supernates and other biological fluids. Detection Range: 1.56-100pg/ml, Sensitivity: <0.66pg/ml, Intra-Assay CV: <10% , Inter-Assay CV: <12%, Kit Components: *028669A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use, *028669B: Standard, 2x1 vial, 028669C: Standard Diluent, 1x20ml, *028669D: Detection Reagent A, 1x120ul, *028669E: Detection Reagent B, 1x120ul, 028669F: Assay Diluent A, 1x12ml, 028669G: Assay Diluent B, 1x12ml, 028669H: TMB Substrate, 1x9ml, 028669K: Stop Solution, 1x6ml, 028669L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (028669K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *028669A, *028669B, *028669D and *028669E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Keywords: TNF, TNFA
Supplier: United States Biological
Supplier-Nr: 028669

Properties

Application: ELISA
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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