TTF1 (Transcription Termination Factor, RNA Polymerase I) BioAssay(TM) ELISA Kit (Human)

Sample Type:, Serum, plasma and other biological fluids. Intended Use: For quantitative detection of TTF1 in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples. Sensitivity: <46.875pg/ml, Precision: Intra-Assay CV: <8%, Inter-Assay CV: <10%, Range: 78.125-5000pg/ml, Specificity: Human, Test Principle: This BioAssay(TM) kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TTF1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TTF1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated d etection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TTF1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TTF1 can be calculated. Kit Components: Microtiter Strips, 1x96 wells (8x12 wells), Standard, 2x1 vial, Sample/Standard Dilution Buffer, 1x20ml, Antibody (Biotin) (Concentrated), 1x120ul, Antibody Dilution Buffer, 1x10ml, Streptavidin (HRP) (SABC), 1x120ul, SABC Dilution Buffer, 1x10ml, TMB Substrate, 1x10ml, Stop Solution, 1x10ml, Wash Buffer, 25X, 1x30ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.