TRAIL BioAssay(TM) ELISA Kit, Human

In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253).Tumor necrosis factor (TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21kD. It is expressed from the amino acids ectoderm T95--G281. Intended Use: TRAIL BioAssay(TM) ELISA Kit, Human is a sandwich ELISA for quantitative detection of human TRAIL in cell culture supernates, cell lysates, serum, plasma (heparin, EDTA) and saliva. Range: 15.6-1000pg/ml, Sensitivity: <1pg/ml, Specificity: Natural and recombinant human TRAIL, Crossreactivity: No detectable crossreactivity with other relevant proteins, Kit Components: 144085A: Microtiter Plate, 1x96 wells, *144085B: TRAIL Standard, 2x1 vial, 144085C: Anti-human TRAIL (Biotin), 1x130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *144085B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or 0.01M PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or 0.01M PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 15-20 minutes., 5. Add TMB stop solution and read.