TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) BioAssay(TM) ELISA Kit (Monkey)

The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD). Sample Type: Serum, cell culture supernates, saliva, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Monkey TIMP-3. Sensitivity: <2pg/ml, Range: 156pg/ml-10,000pg/ml, Specificity: Recognizes natural and recombinant Monkey TIMP-3. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Monkey TIMP-3 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey TIMP-3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey TIMP-3 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Monkey TIMP3 antibody, 1x plate, Lyophilized recombinant Monkey TIMP3 standard, 2x10ng/tube, Biotinylated anti-Monkey TIMP3 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.