THBS1/TSP1 BioAssay(TM) ELISA Kit (Monkey)

Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to Monkey 15q15 and the cognate gene to Monkey chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Monkey THBS1/TSP1. Sensitivity: <50pg/ml, Range: 1.56ng/ml-100ng/ml, Specificity: Recognizes natural and recombinant Monkey THBS1/TSP1. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Monkey THBS1/TSP1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey THBS1/TSP1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey THBS1/TSP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey THBS1/TSP1 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Monkey THBS1 antibody, 1x plate, Lyophilized recombinant Monkey THBS1 standard, 2x100ng/tube, Biotinylated anti-Monkey THBS1 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.