TGF-Beta 2 ELISA Kit (Porcine)

Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured Pig porcine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Porcine TGF-beta 2. Sensitivity: <10pg/ml, Range: 31.2pg/ml-2000pg/ml, Specificity: Recognizes natural and recombinant Porcine TGF-beta 2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Porcine TGF-Beta 2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Porcine TGF- Beta 2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Pig porcine TGF-Beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine TGF-Beta 2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Porcine TGFB2 antibody, 1x plate, Lyophilized recombinant Porcine TGFB2 standard, 2x10ng/tube, Biotinylated anti-Porcine TGFB2 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.