Teratocarcinoma Derived Growth Factor 1 (TDGF1), BioAssay(TM) ELISA Kit (Mouse)

Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of mouse Cripto in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 62.5-4000pg/ml, Specificity: Natural and recombinant mouse Cripto, Test Principle: This mouse Cripto BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cripto has been precoated onto 96-well plates. Standards (NSO, R26-Q150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cripto is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cripto amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse Cripto antibody, 1, Lyophilized recombinant mouse Cripto standard, 10ng/tube×2, Biotinylated mouse Cripto antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, AR1106-3 ABC diluent buffer 12ml, AR1104 TMB color developing agent 10ml, AR1105 TMB stop solution 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.