Surfactant Associated Protein A (SPA) BioAssay(TM) ELISA Kit (Human) (Wide Range)

Sample Type:, Serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids, Intended Use: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SPA in human serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids, Sensitivity: 0.9ng/ml, Range: 2.4-150ng/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Pulmonary Surfactant Associated Protein A1 (SFTPA1). No significant cross-reactivity or interference between Wide-range Pulmonary Surfactant Associated Protein A1 (SFTPA1) and analogues was observed. Precision: Intra-Assay: CV<10% , Inter-Assay: CV<12%, Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to SPA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPA, biotin-conjugated antibody and, enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate , Standard, 2x vial, Detection Reagent A, 1×120ul, Detection Reagent B, 1×120ul, TMB Substrate, 1×9ml, Wash Buffer (30X), 1×20ml, Standard Diluent, 1×20ml, Assay Diluent A, 1×12ml, Assay Diluent B, 1×12ml, Stop Solution, 1x6ml, Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.