sTNFR? BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Soluble TNFR? in cell culture supernates, serum and plasma (heparin, EDTA, citrate and urine. Tumor necrosis factor receptor p75 (TNF-R p75, or TNF-R2) is a 75kD type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor ? (TNFR?) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFR? is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFR? in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFR?, consisting of 24-206 amino acid sequence with the molecular mass of 20kD. , Assay Range:, 7.8pg/ml-500pg/ml, Sensitivity: < 2 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P20333, Entrez Gene ID: 7133, Kit Components: 96-well plate pre-coated with anti-sTNFR?, Human, Lyophilized recombinant sTNFR?, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.