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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
353027.96 | 96 tests | - | - |
3 - 19 business days* |
909.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or... more
Product information "S100 Calcium Binding Protein A9 (S100A9), BioAssay(TM) ELISA Kit (Mouse)"
Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of mouse S100A9 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 31.2-2000pg/ml, Specificity: Natural and recombinant mouse S100A9, Test Principle: This mouse S100A9 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for S100A9 has been precoated onto 96-well plates. Standards(E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse S100A9 amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse S100A9 antibody, 1, Lyophilized recombinant mouse S100A9 standard, 2ng/tube×2, Biotinylated mouse S100A9 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | p14, Cagb, S100a9, MRP-14, Calgranulin-B, Protein S100-A9, S100 calcium-binding protein A9, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14 |
Supplier: | United States Biological |
Supplier-Nr: | 353027 |
Properties
Application: | ELISA |
Species reactivity: | mouse |
Format: | Solid Phase |
Database Information
KEGG ID : | K21128 | Matching products |
UniProt ID : | P31725 | Matching products |
Gene ID | GeneID 20202 | Matching products |
Handling & Safety
Storage: | vT |
Shipping: | +4°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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