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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
027938.96 | 96 tests | - | - |
3 - 19 business days* |
999.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
S100 Calcium Binding Protein A7 (S100A7) BioAssayT ELISA Kit (Human) is a sandwich enzyme... more
Product information "S100 Calcium Binding Protein A7 (S100A7) BioAssay(TM) ELISA Kit (Human)"
S100 Calcium Binding Protein A7 (S100A7) BioAssayT ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A7 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 125-8000pg/ml, Sensitivity: ?49pg/ml, Intra-Assay: CV <10% , Inter-Assay: CV <12%, Kit Components: *027938A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use, *027938B: Standard, 2x1vial , 027938C: Standard Diluent, 1x20ml, *027938D: Detection Reagent A, 1x120ul , *027938E: Detection Reagent B, 1x120ul , 027938F: Assay Diluent A, 1x12ml, 027938G: Assay Diluent B, 1x12ml, 027938H: TMB Substrate, 1x9ml, *027938J: Positive Control, 471.2pg/ml, 1x1vial, 027938K: Stop Solution, 1x6ml, 027938L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (027938K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Notes on 027938J: Positive Control: Intended Use:, The control vial is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contains human S100A7 in human serum (lyophilized powder). Note: The human based material used in this product are screened for HIV, Hepatitis A, B.C, HTLV and Syphilis. Expected Value: 471.2ng/ml, Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4°C and use within 5 days. Storage and Stability: Store *027938A, *027938B, *027938D, *027938E and *027938J at -20°C. After reconstitution of *027938J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, , 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. , 4. Aspirate and wash 3 times. , 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. , 5. Aspirate and wash 5 times., 6. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C. , 7. Add 50ul Stop Solution. Read at 450nm immediately.
Keywords: | PSOR1, S100A7, Psoriasin, Protein S100-A7, S100 calcium-binding protein A7 |
Supplier: | United States Biological |
Supplier-Nr: | 027938 |
Properties
Application: | ELISA |
Species reactivity: | human |
Format: | Solid Phase |
Database Information
KEGG ID : | K21126 | Matching products |
UniProt ID : | P31151 | Matching products |
Gene ID | GeneID 6278 | Matching products |
Handling & Safety
Storage: | vT |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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