Renalase (RNLS) BioAssay(TM) ELISA Kit (Human)

Renalase (RNLS) BioAssayT ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNLS in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 1.25-80ng/ml, Sensitivity: ?0.52ng/ml, Intra-Assay CV: <10% , Inter-Assay CV: <12%, Kit Components: *027847A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use, *027847B: Standard, 2x1 vial , 027847C: Standard Diluent, 1x20ml, *027847D: Detection Reagent A, 1x120ul , *027847E: Detection Reagent B, 1x120ul , 027847F: Assay Diluent A, 2x, 1x6ml, 027847G: Assay Diluent B, 2x, 1x6ml, 027847H: TMB Substrate, 1x9ml, *027847J: Positive Control, 14.67ng/ml, 1x1 vial, 027847K: Stop Solution, 1x6ml, 027847L: Wash Buffer, 30X, 1x20ml , Precaution: The Stop Solution (027847K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027847A, *027847B, *027847D, *027847E and *027847J at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul 027847H: TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul 027847K: Stop Solution. Read at 450nm immediately.