Rat vWF(Von Willebrand Factor) ELISA Kit

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma (By similarity) Assay Type: Sandwich. Sensitivity: 0.043 µg/mL. Standard: 10 µg/mL. Detection range: 0.16-10 µg/mL. Sample type: plasma. Assay length: 3.5h. Research Field: Signal transduction,Hematology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat vWF in the samples is then determined by comparing the OD of the samples to the standard curve.