Rat SST (Somatostatin) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SST in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 15.63-1000 pg/mL. Sensitivity: 5.8 pg/mL. Assay Type: Sandwich. Standard: 1000 pg/mL. Sample Type: sserum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay duration: 3.5h. Protein Function: Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH, the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin , May enhance low-glucose-induced glucagon release by pancreatic alpha cells (PubMed:24735892). This effect may be mediated by binding to GPR107 and PKA activation (PubMed:26561648). May regulate cardiac contractile function (PubMed:22170057). May compromise cardiomyocyte viability (PubMed:22170057). In the central nervous system, may impair memory retention and may affect hippocampal excitability (PubMed:21978882). May also have anxiolytic and anorexigenic effects (PubMed:18753129, PubMed:21978882). May play a role in arterial pressure regulation (PubMed:18753129, PubMed:21978882). May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH (By similarity). Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH (By similarity) [The Uniprot Consortium]