Rat NUCB2 (Nucleobindin 2) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NUCB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NUCB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NUCB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NUCB2 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 0.78-50 ng/mL. Sensitivity: 0.34 ng/mL. Assay Type: Sandwich. Standard: 50 ng/mL. Sample Type: sSerum, plasma and other biological fluids.. Assay duration: 3.5h. Protein Function: Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (PubMed:21653697) , Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. In intestinal epithelial cells, plays a role in the inhibition of hepatic glucose production via MC4R receptor leading to increased cyclic adenosine monophosphate (cAMP) levels and glucagon-like peptide 1 (GLP-1) secretion (By similarity) [The Uniprot Consortium]