Rat NT5E (5'-Nucleotidase, Ecto) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT5E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT5E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT5E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT5E in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:1770988). AMP is the preferred substrate but can also hydrolyze CMP and GMP (PubMed:1770988). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Other substrates include IMP, UMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity) [The Uniprot Consortium]