Rat Haptoglobin, Hpt/HP ELISA KIT

Sample Types: serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine Detection Range: 0.156 µg/mL-10 µg/mL Sensitivity: 0.039 µg/mL Assay Principle: quantitative Measurement: SandwichAssay Time: 1-5h Sample Volume: 50-100ulDetection Wavelength: 450 nmProtein function: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. [The UniProt Consortium]