Rat CA15-3 (Carbonhydrate Antigen 15-3) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 7.82-500 pg/mL. Sensitivity: 3.74 pg/mL. Assay Type: Sandwich. Standard: 500 pg/mL. Sample Type: sSerum, plasma, tissue homogenates, cell lysates and other biological fluids. Assay duration: 3.5h.