R-Spondin-3 BioAssay(TM) ELISA Kit (Mouse)

R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the R- spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse R-Spondin-3. Sensitivity: <10pg/ml, Range: 15.6pg/ml-1000pg/ml, Specificity: Recognizes natural and recombinant Mouse R-Spondin-3. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse R-Spondin-3 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for R-Spondin-3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q22-H277) and test samples are added to the wells, a biotinylated detection specific for R-Spondin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse R-Spondin-3 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Rspo antibody, 1x plate, Lyophilized recombinant Mouse Rspo standard, 2x10ng/tube, Biotinylated anti-Mouse Rspo antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.