Prostaglandin Screening ELISA Kit

This assay was developed for screening applications in which the relative amount of prostaglandin (PG) production for a large number of cell culture samples must be determined. Our PGE2 assay (Cay-514010) is unparalleled in sensitivity and specificity, but the steep standard curve can make screening many samples tedious. The specificity of the PGE2 assay also eliminates quantification of other PGs which may be produced by the cells. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given sample with a single assay. The broad standard curve minimizes the need for sample dilution. Because this assay will also recognize thromboxane B2 (TXB2), cell culture media must not contain fetal calf serum (FCS). The high concentration of TXB2 in serum (approximately 400 ng/ml) will completely displace the tracer, making PG measurement impossible.Synonyms: PG Screening EIA Kit. Assay Range: 15.6-2,000 pg/ml. Sensitivity: 80% B/B0: 30 pg/ml. Mid-point: 50% B/B0: 125-250 pg/ml. Cross Reactivity: (Prostaglandin E1) 100%, (Prostaglandin E2) 100%, (Prostaglandin F1alpha) 100%, (Prostaglandin F2alpha) 100%, (Prostaglandin F3alpha) 51.3%, (Prostaglandin E2 Ethanolamide) 44.0%, (6-keto Prostaglandin F1alpha) 43.6%, (8-iso Prostaglandin F2alpha) 38.4%, (8-iso Prostaglandin E2) 28.5%, (Prostaglandin D2) 26.6%, (8-iso-2,3-dinor Prostaglandin F1alpha) 20.0%, (Prostaglandin E3) 9.5%, (Thromboxane B2) 5.0%, (12(S)-HHTrE) 0.25%, (Leukotriene D4) 0.2%, (Arachidonic Acid) <0.01%, (Leukotriene B4) <0.01%, (Leukotriene C4) <0.01%, (Leukotriene E4) <0.01%, (Misoprostol) <0.01%, (Misoprostol (free acid)) <0.01%, (Prostaglandin A1) <0.01%, (Prostaglandin A2) <0.01%, (Prostaglandin A3) <0.01%, (Prostaglandin B1) <0.01%, (15-keto Prostaglandin E2) <0.01%, (13,14-dihydro-15-keto Prostaglandin F2alpha) <0.01%. Origin: Animal/Rabbit, Animal/Mouse, Animal/Bovine, Animal/Eel.