Osteoprotegerin (OPG) BioAssay(TM) ELISA Kit (Human)

This protein is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. Sample Type: Serum, plasma, tissue homogenates, Intended Use: For the quantitative determination of human osteoprotegerin (OPG) concentrations in serum, plasma, tissue homogenates. Sensitivity: 0.078ng/ml, Range: 7.8-500pg/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of human OPG. No significant cross-reactivity or interference between human OPG and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPG bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells, Standard (Freeze dried), 2x vials, Biotin-antibody (100X), 1x120ul, HRP-avidin (100X), 1x120ul, Biotin-antibody Diluent, 1x15ml, HRP-avidin Diluent, 1x15ml, Sample Diluent, 1x50ml, Wash Buffer (25X), 1x20ml, TMB Substrate, 1x10ml, Stop Solution, 1x10ml, Adhesive Strip (For 96 wells), 4x strips, Instruction manual, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.