Mouse REG3b (Regenerating Islet Derived Protein 3 Beta) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse REG3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse REG3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse REG3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse REG3b in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Bactericidal C-type lectin which acts against several intestinal Gram-positive and Gram-negative bacteria. Lacks antibacterial activity against S.typhimurium. May play a role in protection against infection with S.enteritidis by inhibiting its translocation from the gut lumen into intestinal tissues and further extraintestinal tissues , Acts as a hormone in response to different stimuli. Secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways (PubMed:34099862). In pancreas, is able stimulate cell proliferation (PubMed:34099862) [The Uniprot Consortium]