Mouse PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIGF in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 15.6-1000 ng/mL. Sensitivity: 6.3 ng/mL. Assay Type: Competitive Inhibition. Standard: 1000 ng/mL. Sample Type: sSerum, plasma, tissue homogenates and other biological fluids. Assay duration: 2h. Protein Function: Stabilizing subunit of the ethanolamine phosphate transferase 3 and ethanolamine phosphate transferase 2 complexes that sequentially transfer an ethanolamine phosphate (EtNP) from a phosphatidylethanolamine (PE) to the 6-OH position of the third alpha-1,2-linked mannose and the second alpha-1,6-linked mannose of the alpha-D-Man-(1->2)-alpha-D-Man-(1->6)-2-PEtn-alpha-D-Man-(1->4)-alpha-D-GlcN-(1->6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H6) intermediate to generate a 6-PEtn-alpha-D-Man-(1->2)-6-PEtn-alpha-D-Man-(1->6)-2-PEtn-alpha-D-Man-(1->4)-alpha-D-GlcN-(1->6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H8) (PubMed:10781593). Participates in the tenth and eleventh steps of the glycosylphosphatidylinositol-anchor biosynthesis, in association with PIGO and PIGG, respectively (PubMed:10781593) [The Uniprot Consortium]