Mouse MTTP (Microsomal Triglyceride Transfer Protein) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MTTP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MTTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MTTP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MTTP in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces (PubMed:15897609, PubMed:16478722, PubMed:18502767, PubMed:33168624). Required for the assembly and secretion of plasma lipoproteins that contain apolipoprotein B (PubMed:10713055, PubMed:17635917, PubMed:18502767, PubMed:33168624, PubMed:9502759). May be involved in regulating cholesteryl ester biosynthesis in cells that produce lipoproteins (PubMed:18502767) , Critical for the development of natural killer T (NKT) cells (PubMed:17312007). Required for the assembly and secretion of plasma lipoproteins that contain apolipoprotein B (PubMed:17635917) [The Uniprot Consortium]