Mouse MRE11 (Double-strand break repair protein MRE11) ELISA Kit

Mouse MRE11 (Double-strand break repair protein MRE11) ELISA Kit is a high quality kit for research use only from Assay Genie. The Mouse MRE11 (Double-strand break repair protein MRE11) ELISA Kit comes in a 96 Assays format and reacts against Mouse samples. We ship this kit on Gel Pack and kits should be stored at 2-8°C Protein Function: Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:14690604, PubMed:17291760, PubMed:18854157). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:18854157). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:14690604, PubMed:18854157). Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity (PubMed:18854157). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with RBBP8/CtIP to initiate end resection (By similarity). MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR (By similarity). It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation to create entry sites for EXO1- and DNA2-mediated 5' to 3' long-range resection, which is required for single-strand invasion and recombination (By similarity). RBBP8/CtIP specifically promotes the endonuclease activity of MRE11 to clear protein-DNA adducts and generate clean double-strand break ends (By similarity). MRE11 endonuclease activity is also enhanced by AGER/RAGE (PubMed:28977635). The MRN complex is also required for DNA damage signaling via activation of the ATM and ATR kinases: the nuclease activity of MRE11 is not required to activate ATM and ATR (PubMed:18854157). The MRN complex is also required for the processing of R-loops (By similarity). The MRN complex is involved in the activation of the cGAS-STING pathway induced by DNA damage during tumorigenesis: the MRN complex acts by displacing CGAS from nucleosome sequestration, thereby activating it (PubMed:38200309). In telomeres the MRN complex may modulate t-loop formation (By similarity) [The Uniprot Consortium]