Mouse KLK1(Kallikrein 1) ELISA Kit

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin Assay Type: Competitive Inhibition. Sensitivity: 0.14 ng/mL. Standard: 20 ng/mL. Detection range: 0.32-20 ng/mL. Sample type: Serum, plasma and other biological fluids.. Assay length: 2.5h. Research Field: Enzyme & Kinase,Tumor immunity,Hepatology,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.