Mouse IL-7 ELISA Kit

Interleukin-7 (IL-7) is a protein that is encoded by the IL-7 gene in humans. The gene for human interleukin-7 (IL-7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization. IL-7 is essential for survival and expansion of pathogenic T helper type 17 (Th17) cells in experimental autoimmune encephalomyelitis (EAE). ScienCell's mouse IL-7 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse IL-7-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse IL-7 in the sample that is captured on the strips.