Mouse GREM2(Gremlin 2) ELISA Kit

Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells Assay Type: Sandwich. Sensitivity: 12.2 pg/mL. Standard: 2000 pg/mL. Detection range: 31.25-2000 pg/mL. Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h. Research Field: Signal transduction,Tumor immunity,Developmental science,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GREM2 in the samples is then determined by comparing the OD of the samples to the standard curve.