Mouse GDF11 (Growth Differentiation Factor 11) ELISA (Small Sample Volume)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDF11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDF11 in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Secreted signal that acts globally to regulate anterior/posterior axial patterning during development (PubMed:10391213). May play critical roles in patterning both mesodermal and neural tissues (PubMed:10391213). It is required for proper vertebral patterning and orofacial development (By similarity). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed:12414726, PubMed:16845371) [The Uniprot Consortium]