Mouse FOLR1 (Folate Receptor 1, Adult) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FOLR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FOLR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FOLR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FOLR1 in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:1894617). Has high affinity for folate and folic acid analogs at neutral pH (By similarity). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (By similarity). Required for normal embryonic development and normal cell proliferation (PubMed:10508523, PubMed:12854656, PubMed:15259034, PubMed:17286298). Required for renal folate reabsorption (PubMed:15703271) [The Uniprot Consortium]