Mouse CyPC(Peptidylprolyl Isomerase C) ELISA Kit

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding Assay Type: Sandwich. Sensitivity: 12.1 pg/mL. Standard: 2000 pg/mL. Detection range: 31.25-2000 pg/mL. Sample type: serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids. Assay length: 3.5h. Research Field: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CyPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CyPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CyPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CyPC in the samples is then determined by comparing the OD of the samples to the standard curve.