Mouse CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (By similarity). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (PubMed:11970866, PubMed:21728997). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (PubMed:30566056) Assay Type: Sandwich. Sensitivity: 0.049 ng/mL. Standard: 10 ng/mL. Detection range: 0.16-10 ng/mL. Sample type: Tissue homogenates and other biological fluids.. Assay length: 3.5h. Research Field: Epigenetics and Nuclear Signaling, Immunology, Neuroscience. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CREB in the samples is then determined by comparing the OD of the samples to the standard curve.