Mouse a1ACT (Alpha-1-Antichymotrypsin) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse a1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse a1ACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1ACT in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 15.63-1000 µg/mL. Sensitivity: 5.01 µg/mL. Assay Type: Sandwich. Standard: 1000 µg/mL. Sample Type: sserum, plasma and other biological fluids. Assay duration: 3.5h. Protein Function: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2 [The Uniprot Consortium]