Melanoma Cell Adhesion Molecule (MCAM) BioAssay(TM) ELISA Kit (Human)

Melanoma Cell Adhesion Molecule (MCAM) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human MCAM in serum, plasma and other biological fluids. Detection Range: 31.2-2000ng/ml, Sensitivity: 1.44ng/ml, Intra-Assay: CV<10%, Inter-Assay: CV<12%, Kit Components: *026770A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use, *026770B: Standard, 2x1vial , 026770C: Standard Diluent, 1x20ml, *026770D: Detection Reagent A, 1x120ul , *026770E: Detection Reagent B, 1x120ul , 026770F: Assay Diluent A, 1x12ml, 026770G: Assay Diluent B, 1x12ml, 026770H: TMB Substrate, 1x9ml, 026770K: Stop Solution, 1x6ml, 026770L: Wash Buffer, 30X, 1x20ml, Storage and Stability: Store *026770A, *026770B, *026770D and *026770E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.