IP3 (Inositol Triphosphate) BioAssay(TM) ELISA Kit

This Inositol Triphosphate (IP3) BioAssay(TM) ELISA Kit is a competitive ELISA for the in vitro quantitative determination of IP3 concentrations in serum, plasma and other biological fluids. Sensitivity: <0.094ng/ml, Precision: Intra-Assay CV: <8%, Inter-Assay CV: <10%, Range: 0.156-10ng/ml, Specificity: Universal, Test Principle: The microtiter plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the sample or standard competes with a fixed amount of IP3 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to IP3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of IP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: Microtiter Strips, 1x96 wells (8x12 wells), Standard, 2x1 vial, Sample/Standard Dilution Buffer, 1x20ml, Antibody (Biotin) (Concentrated), 1x120ul, Antibody Dilution Buffer, 1x10ml, Streptavidin (HRP) (SABC), 1x120ul, SABC Dilution Buffer, 1x10ml, TMB Substrate, 1x10ml, Stop Solution, 1x10ml, Wash Buffer, 25X, 1x30ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.