Interleukin 5, Human, BioAssay(TM) ELISA Kit (IL-5, Interleukin-5, B Cell Differentiation Factor I,

Sandwich ELISA quantitative detection kit for Human IL-5 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology. , Detection Range:, 7.8-500pg/ml, Sensitivity: < 2pg/ml, Intra-Assay: CV<10%, Inter-Assay: CV<10%, Specificity: Natural and recombinant human IL-5, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P05113, Entrez Gene ID: 3567, Kit Components: 143927A: Microtiter Strip, 1x96 wells, *143927B: IL-5 Standard, 2x10ng, 143927C: Anti-human IL-5 (Biotin), 130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143927B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.