IL1RL1:IL33 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The IL1RL1: IL33 [Biotinylated] Inhibitor Screening Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between IL-1RL1 (interleukin 1 receptor like 1 or IL33R) and IL-33 protein for screening and profiling applications. This kit comes with enough purified IL1RL1 (amino acids 19-328) and biotinylated IL33 (amino acids 109-270), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. IL1RL1: IL33 [Biotinylated] Inhibitor Screening Chemiluminescent Assay Kit schematic. A 96-well plate is coated with IL1RL1 protein. After coating and blocking, biotinylated IL33 is added in an optimized assay buffer. Next, unbound biotinylated IL33 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL33 binding to IL1RL1. Need us to run inhibitor screens or profile your compounds that block the binding og IL1RL1 to IL33? Check out our Immunotherapy Screening Services.