IL-8 BioAssay(TM) ELISA Kit, Human

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils (1). IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications (2). The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside (1). Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form. Intended Use: IL-8 BioAssay(TM) ELISA Kit, Human is a sandwich ELISA for quantitative detection of human IL-8 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Range: 15.6-1000pg/ml (cell culture supernates), 7.8-500pg/ml (serum, plasma), Sensitivity: < 1pg/ml, Specificity: Natural and recombinant human IL-8, Crossreactivity: No detectable crossreactivity with other relevant proteins, Kit Components: 143935A: Microtiter Strips, 1x96 wells, 143935B: IL-8 Standard, 2x10ng, 143935C: Anti-human IL-8 (Biotin), 1x130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5times with 0.01M TBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes., 5. Add TMB stop solution and read.. Storage and Stability: Store *143935B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.