IL-7 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Interleukin 7 in cell culture supernates, serum and plasma (EDTA). IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4 . Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6?7?8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. , Assay Range:, 15.6pg/ml-1000pg/ml, Sensitivity: < 1 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: A8K673, Entrez Gene ID: 3574, Kit Components: 96-well plate pre-coated with anti-IL-7, Human, Lyophilized recombinant IL-7, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.