IL-6R alpha BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-6R alpha in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution. , Assay Range:, 31.2pg/ml-2000pg/ml, Sensitivity: < 10 pg/ml , Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: A0N0L5, Entrez Gene ID: 3570, Kit Components: 96-well plate pre-coated with anti-IL-6R alpha, Human, Lyophilized recombinant IL-6R alpha, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.