IL-21 (Human) Chemiluminescent ELISA Detection Kit

The IL-21 (Human) Chemiluminescent ELISA Detection Kit is a sandwich ELISA designed for detecting and quantifying human interleukin-21 (IL-21) in cell culture medium. This kit comes with enough anti-IL-21 capture and detection antibodies, IL-21 standard, and detection reagents for 100 or 5 x 100 enzyme reactions. Figure 1. IL-21 (Human) Chemiluminescent ELISA Detection Kit schematic. A 96-well plate is coated with an anti-IL-21 capture antibody. After coating and blocking, samples containing IL-21 are added. Next, unbound IL-21 is washed away, and the plate is incubated with biotinylated IL-21 detection antibody. After another wash, streptavidin-HRP is added. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the amount of IL-21 in the samples. IL-21 (interleukin-21) is a cytokine mainly produced by CD4+ T cells that triggers effector function and memory differentiation of CD8+ T cells during infection and in cancer (such as Hodgkin's lymphoma). IL-21 expression is also found Iin NKT cells, where it regulates their function. It binds to the receptor IL-21R, found on the surface of T, B and NK cells. In B cells it leads to the generation of high-affinity class-switched antibodies. The functional signaling complex activates Janus kinases JAK1, JAK3, and the STAT (signal transducer and activator of transcription) proteins STAT1 and STAT3, impacting cell survival and proliferation. High levels of IL-21, expression of IL21R and polymorphisms in both IL-21 and IL21R have been described in autoimmune diseases. The development of therapeutic strategies targeting IL-21 will impact patients with SLE (systemic lupus erythematosus), type I diabetes, MS (multiple sclerosis), IBD (inflammatory bowel disease), and psoriasis.