IGFBP7 BioAssay(TM) ELISA Kit, Human

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers. Intended Use: For quantitative detection of human IGFBP7 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Range: 625-40,000pg/ml, Sensitivity: < 20pg/ml, Specificity: Natural and recombinant human IGFBP7, Crossreactivity: No detectable crossreactivity with other relevant proteins, Kit Components: 143881A: Microtiter Strips, 1x96 wells, 143881B: IGFBP7 Standard, 2x40ng, 143881C: Anti-human IGFBP7 (Biotin), 1x130ul, 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143881B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 min. Do not wash., 2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min., 5. Add 100ul TMB stop solution and read.