Human Survivin ELISA Kit

Survivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers, including lung, colon, pancreas, prostate, and breast cancer in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein sequence and localizes to chromosome 17q25. The overexpression of it in cancer may allow cancer cells to overcome an apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with a molecular mass of 34 kDa. ScienCell's human Survivin ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Survivin-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human Survivin in the sample that is captured on the strips.