Human Resistin ELISA Kit

Resistin is an adipose-derived hormone, postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signaling molecule, Resistin (resistance to insulin), that may potentially link obesity to diabetes. Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance. The standard product used in this kit is human Resistin with a molecular mass of 51-53 kDa. The detected Resistin includes zymogen and active enzyme. ScienCell's human Resistin ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Resistin-specific polyclonal antibodies are pre-coated onto 96-well plates. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human Resistin in the sample that is captured on the strips.