Human Rantes ELISA Kit

Rantes (from "regulated on activation normal T cell expressed and secreted"), also known as CCL5, is one of the natural ligands for the chemokine receptor CCR5.It potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a co-receptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete Rantes. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag- specific and growth factor dependent. ScienCell's human Rantes ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Rantes-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human Rantes captured in strips.