Human PRG4 (Proteoglycan 4) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRG4 in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface , Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages [The Uniprot Consortium]