Human OC (Osteocalcin) ELISA Kit

Human OC (Osteocalcin) ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ELK-ELK2390.48 48 wells - -

10 - 14 business days*

365.00€
ELK-ELK2390.96 96 wells - -

10 - 14 business days*

522.00€
 
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate... more
Product information "Human OC (Osteocalcin) ELISA Kit"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 0.63-40 ng/mL. Sensitivity: 0.252 ng/mL. Assay Type: Sandwich. Standard: 40 ng/mL. Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay duration: 3.5h
Keywords: BGP, BGLAP, Osteocalcin, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein
Supplier: ELK Biotechnology
Supplier-Nr: ELK2390

Properties

Application: ELISA
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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