Human LILRA3 (Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3) ELISA (Small Sample Volu

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LILRA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LILRA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LILRA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LILRA3 in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Acts as a soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes [The Uniprot Consortium]