Human LAMR1 (Laminin Receptor 1) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAMR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAMR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAMR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAMR1 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 78.13-5000 pg/mL. Sensitivity: 29 pg/mL. Assay Type: Sandwich. Standard: 5000 pg/mL. Sample Type: stissue homogenates, cell lysates and other biological fluids. Assay duration: 3.5h. Protein Function: Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA , (Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9 , (Microbial infection) Acts as a receptor for the Dengue virus , (Microbial infection) Acts as a receptor for the Sindbis virus , (Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus , (Microbial infection) Acts as a receptor for the pathogenic prion protein , (Microbial infection) Acts as a receptor for bacteria [The Uniprot Consortium]